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anti post synaptic density 95 psd95  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc anti post synaptic density 95 psd95
    Anti Post Synaptic Density 95 Psd95, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 652 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+post+synaptic+density+95/PSD95+XP+Rabbit+mAb/pm40076425-252-51-60
    Average 96 stars, based on 652 article reviews
    anti post synaptic density 95 psd95 - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Membrane:

    Article Title: NGF‐Dependent Activation of TrkA Pathway: A Mechanism for the Neuroprotective Effect of Troxerutin in D‐Galactose‐Treated Mice
    Article Snippet: Samples (80 μg protein each) were separated by denaturing SDS‐PAGE and transferred to a PVDF membrane (Roche Diagnostics Corporation) by electrophoretic transfer. .. The membrane was blocked with 5% nonfat milk and 0.1% Tween‐20 in TBS, incubated overnight with monoclonal rabbit anti‐Akt (pThr 308 ) antibody (1:1000, Cell Signaling Technology, Inc.) or monoclonal rabbit anti‐phospho‐cyclic AMP response element‐binding protein [anti‐CREB (pSer 133 )] antibody (1:1000, Cell Signaling Technology, Inc.) or polyclonal rabbit anti‐phospho‐α subunit of calcium/calmodulin‐dependent protein kinase II [anti‐CaMKII‐α (pThr 286 )] antibody (1:1000, Santa Cruz Biotechnology, CA, USA) or monoclonal rabbit anti‐phospho‐extracellular signal‐regulated kinase 1 and 2 [anti‐ERK1/2 (pThr 202 /Tyr 204 )] antibody (1:1000, Cell Signaling Technology, Inc.) or monoclonal rabbit anti‐ERK1/2 antibody (1:1000, Cell Signaling Technology, Inc.), or monoclonal mouse anti‐post‐synaptic density‐95 (anti‐PSD95) antibody (1:1000, Abcam, Cambridge, UK), respectively. .. Quantitation of detected bands was performed with the Scion Image analysis software (Scion Corp.).

    Incubation:

    Article Title: NGF‐Dependent Activation of TrkA Pathway: A Mechanism for the Neuroprotective Effect of Troxerutin in D‐Galactose‐Treated Mice
    Article Snippet: Samples (80 μg protein each) were separated by denaturing SDS‐PAGE and transferred to a PVDF membrane (Roche Diagnostics Corporation) by electrophoretic transfer. .. The membrane was blocked with 5% nonfat milk and 0.1% Tween‐20 in TBS, incubated overnight with monoclonal rabbit anti‐Akt (pThr 308 ) antibody (1:1000, Cell Signaling Technology, Inc.) or monoclonal rabbit anti‐phospho‐cyclic AMP response element‐binding protein [anti‐CREB (pSer 133 )] antibody (1:1000, Cell Signaling Technology, Inc.) or polyclonal rabbit anti‐phospho‐α subunit of calcium/calmodulin‐dependent protein kinase II [anti‐CaMKII‐α (pThr 286 )] antibody (1:1000, Santa Cruz Biotechnology, CA, USA) or monoclonal rabbit anti‐phospho‐extracellular signal‐regulated kinase 1 and 2 [anti‐ERK1/2 (pThr 202 /Tyr 204 )] antibody (1:1000, Cell Signaling Technology, Inc.) or monoclonal rabbit anti‐ERK1/2 antibody (1:1000, Cell Signaling Technology, Inc.), or monoclonal mouse anti‐post‐synaptic density‐95 (anti‐PSD95) antibody (1:1000, Abcam, Cambridge, UK), respectively. .. Quantitation of detected bands was performed with the Scion Image analysis software (Scion Corp.).



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    List of primary antibodies used for Western blotting and immunohistochemistry
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    Image Search Results


    List of primary antibodies used for Western blotting and immunohistochemistry

    Journal: In Vitro Cellular & Developmental Biology. Animal

    Article Title: Differentiation of SH-SY5Y neuroblastoma cells using retinoic acid and BDNF: a model for neuronal and synaptic differentiation in neurodegeneration

    doi: 10.1007/s11626-024-00948-6

    Figure Lengend Snippet: List of primary antibodies used for Western blotting and immunohistochemistry

    Article Snippet: Post-synaptic density-95 (PSD-95) (D27E11) , Rabbit monoclonal , Cell Signaling technologies , 3450 , 1:500.

    Techniques: Western Blot, Recombinant

    Time-dependent expression of key neuronal and synaptic markers during differentiation of SH-SY5Y cells. All experiments involved SDS-PAGE and Western blotting of whole cell lysates at the designated timepoints. ( A ): Expression of β-III tubulin. Top : quantification of the relative expression levels normalised to D10. Bottom : representative Western blot. ( B ): Expression of synaptophysin. Top : quantification of the relative expression levels normalised to D10. Bottom : representative Western blot. ( C ): Expression of PSD-95. Top : quantification of the relative expression levels normalised to D10. Bottom : representative Western blot. For all graphs , values are mean ± SEM ( n = 3). * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. 1-way ANOVA with Tukey’s post-hoc test.

    Journal: In Vitro Cellular & Developmental Biology. Animal

    Article Title: Differentiation of SH-SY5Y neuroblastoma cells using retinoic acid and BDNF: a model for neuronal and synaptic differentiation in neurodegeneration

    doi: 10.1007/s11626-024-00948-6

    Figure Lengend Snippet: Time-dependent expression of key neuronal and synaptic markers during differentiation of SH-SY5Y cells. All experiments involved SDS-PAGE and Western blotting of whole cell lysates at the designated timepoints. ( A ): Expression of β-III tubulin. Top : quantification of the relative expression levels normalised to D10. Bottom : representative Western blot. ( B ): Expression of synaptophysin. Top : quantification of the relative expression levels normalised to D10. Bottom : representative Western blot. ( C ): Expression of PSD-95. Top : quantification of the relative expression levels normalised to D10. Bottom : representative Western blot. For all graphs , values are mean ± SEM ( n = 3). * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. 1-way ANOVA with Tukey’s post-hoc test.

    Article Snippet: Post-synaptic density-95 (PSD-95) (D27E11) , Rabbit monoclonal , Cell Signaling technologies , 3450 , 1:500.

    Techniques: Expressing, SDS Page, Western Blot

    Punctate distribution of pre- and post-synaptic markers along neurites of D10 differentiated SH-SY5Y cells. ( A-C ): Immunocytochemistry for DAPI ( blue , A ), synaptophysin ( green , B ) and merged ( C ) in SH-SH5Y cells after 10 d of differentiation, showing diffuse somatic and more punctate neurite localisation. Images are the composite of 33 confocal slices. ( D-F ): Immunocytochemistry for DAPI ( blue , D ), PSD-95 ( green , E ) and merged ( F ) in SH-SH5Y cells after 10 d of differentiation, showing diffuse somatic and more punctate neurite localisation. ( G-I ): Immunocytochemistry for PSD-95 ( green , G ), synaptophysin ( red , H ) and merged ( I ). Arrows in I indicate areas of close apposition of PSD-95 and synaptophysin staining. Images are the composite of 32 confocal slices. Scale bar = 50 µM.

    Journal: In Vitro Cellular & Developmental Biology. Animal

    Article Title: Differentiation of SH-SY5Y neuroblastoma cells using retinoic acid and BDNF: a model for neuronal and synaptic differentiation in neurodegeneration

    doi: 10.1007/s11626-024-00948-6

    Figure Lengend Snippet: Punctate distribution of pre- and post-synaptic markers along neurites of D10 differentiated SH-SY5Y cells. ( A-C ): Immunocytochemistry for DAPI ( blue , A ), synaptophysin ( green , B ) and merged ( C ) in SH-SH5Y cells after 10 d of differentiation, showing diffuse somatic and more punctate neurite localisation. Images are the composite of 33 confocal slices. ( D-F ): Immunocytochemistry for DAPI ( blue , D ), PSD-95 ( green , E ) and merged ( F ) in SH-SH5Y cells after 10 d of differentiation, showing diffuse somatic and more punctate neurite localisation. ( G-I ): Immunocytochemistry for PSD-95 ( green , G ), synaptophysin ( red , H ) and merged ( I ). Arrows in I indicate areas of close apposition of PSD-95 and synaptophysin staining. Images are the composite of 32 confocal slices. Scale bar = 50 µM.

    Article Snippet: Post-synaptic density-95 (PSD-95) (D27E11) , Rabbit monoclonal , Cell Signaling technologies , 3450 , 1:500.

    Techniques: Immunocytochemistry, Staining